Publications

1975
Bhagwat, V.M. & Ramachandran, B.V. Malathion A and B esterases of mouse liver-I. Biochem Pharmacol 24, 18, 1713-7 (1975).
Randerson, James, T. & Slotkin, T.A. Maturation of the adrenal medulla--IV. Effects of morphine. Biochem Pharmacol 24, 16, 1469-74 (1975).
Smith, R.J. & Bryant, R.G. Metal substitutions incarbonic anhydrase: a halide ion probe study. Biochem Biophys Res Commun 66, 4, 1281-6 (1975).
Lorand, L., Shishido, R., Parameswaran, K.N. & Steck, T.L. Modification of human erythrocyte ghosts with transglutaminase. Biochem Biophys Res Commun 67, 3, 1158-66 (1975).
Sadler, A.M. New health practitioner education: problems and issues. J Med Educ 50, 12 pt 2, 67-73 (1975).
Mamytov, A.M. [Organization of medical aid to patients with mandibular fractures in the Kirghiz SSR]. Zdravookhr Kirg 3, 7-9 (1975).
Imai, K. & Lehmann, H. The oxygen affinity of haemoglobin Tak, a variant with an elongated beta chain. Biochim Biophys Acta 412, 2, 288-94 (1975).Abstract
The oxygen affinity was investigated of purified Hb Tak, a human haemoglobin variant with elongated beta-chains. A very low P50 value was found which was not influenced by the addition of 2,3 diphosphoglycerate. The n value was 1, indicating non-cooperativity. The oxygen equilibrium curve of the whole blood haemolysate containing Hbs A and Tak was close to that of Hb A at the top of the curve, while the bottom of the curve greatly deviated from the latter, indicative of small if any interaction between Hb A and Tak during oxygenation.
Motoki, H., Kurokawa, T., Shiraishi, H., Kitame, F. & Ono, Y. Purification of an immunosuppressive principle from Ehrlich carcinoma ascites. Gan 66, 5, 569-72 (1975).Abstract
An immunosuppressive factor was purified from Ehrlich carcinoma ascites by the combination of ultrafiltration and Sephadex chromatography. The resulting product showed 50% reduction in the number of splenic plaque-forming cells in mice immunized with sheep red blood cells when as low as 50 mug dose was given twice intraperitoneally before erythrocyte injection. The molecular size of the product was between 30,000 and 100,000, and it was relatively heat unstable.
Nürnberger, H. [Quantitative colorimetric determination of 1-(4-cyclohexylureidophenoxy)-2-hydroxy-3-tert butylaminopropane (talinolol Cordanum, 02-115) in body fluids]. Pharmazie 30, 10, 673 (1975).
Marniemi, J. & Parkki, M.G. Radiochemical assay of glutathione S-epoxide transferase and its enhancement by phenobarbital in rat liver in vivo. Biochem Pharmacol 24, 17, 1569-72 (1975).
Nourse, E.S. The regional workshops on primary care. J Med Educ 50, 12 pt 2, 201-9 (1975).
Zourlas, P.A. Response to exogenous gonadotropins in the unresponsive ovary syndrome. Int J Gynaecol Obstet 13, 1, 23-8 (1975).
Chow, Y.W., Pietranico, R. & Mukerji, A. Studies of oxygen binding energy to hemoglobin molecule. Biochem Biophys Res Commun 66, 4, 1424-31 (1975).
Scherberger, R.R., Kaess, H. & Brückner, S. [Studies on the action of an anticholinergic agent in combination with a tranquilizer on gastric juice secretion in man]. Arzneimittelforschung 25, 9, 1460-3 (1975).Abstract
A double-blind study with intra-individual comparisons was carried out to investigate the effects of 15 mg of (8r)-3alpha-hydroxy-8-isopropyl-1alphaH-tropanium bromide(+/-)-tropate (Sch 1000), 15 mg Sch 1000 + 10 mg oxazepam, 10 mg oxazepam and placebo with oral administration in randomized sequence on gastric juice volume, amount of acid, concentration and pH values in 12 healthy volunteers. The secretion parameters were measured during a 1-h basal period and a 2-h stimulation period. The gastric juice was obtained in 15 min portions via stomach tube. Stimulation was effected by 1 mug/kg/h pentagastrin via drip infusion. The Friedman test was used for the comparative statistical evaluation, and individual comparisons were carried out by means of the Wilcoxon test (pair-differences rank). The results show that Sch 1000 and Sch 1000 + oxazepam were equal in effect on basal and stimulated secretion volume. As compared with placebo, it was not possible to establish an effect on secretion volume for oxazepam alone. Sch 1000 and Sch 1000 + oxazepam were found to be equipotent in reducing the amount of basal acid, while oxazepam reduced this quantity only during the first 30 min of basal secretion. None of the three active preparations was capable of inhibiting the stimulated acid, although both Sch 1000 preparations produced a clear trend towards lowered mean values. During the basal secretion period, all three test preparations had an inhibiting action on acid concentration, but none of them had a significant effect during the stimulation period. The pH value was savely increased only by Sch 1000 and Sch 1000 + oxazepam, and this even only during the basal period. The results are discussed.
Verbruggen, R. Subtilopeptidase A isoenzyme system. Interaction with serum components and its importance for quantitative immunoelectrophoresis. Biochem J 151, 1, 149-55 (1975).Abstract
A method was developed which involved electroimmunoassay and crossed immunoelectrophoresis of subtilopeptidase A (EC 3.4.21.14). Initial trials with unfractionated antiserum were not successful and interaction of the enzyme with non-immunoglobulin serum components were shown to be the cause of the failures. Quantitative immunoelectrophoresis was possible when purified immunoglobulins were used. A pH of 6.5 (lower than the usual pH 8.6) was necessary to obtain a proper baseline definition. Subtilopeptidase A was confirmed as a multiple isoenzyme system. Qualitative inter-batch variations were detected. Di-isopropyl phosphorofluoridate inhibition altered the electrophoretic pattern, but no loss of antigenic determinants was observed.
Vasina, I.G., Frolov, E.P. & Serebriakov, N.G. [Sympathico-adrenal system activity in a primary immune response]. Zh Mikrobiol Epidemiol Immunobiol 9, 88-92 (1975).Abstract
Experiments were carried out on linear mice immunized with sheep erythrocytes; it was found that the primary immune respose developed against the background of significant changes in the state of the sympathico-adrenal system, whose activity was determined by the dynamics of catecholamines in the blood and in the tissues of a number of organs, including the thymus, the spleen and the lymph nodes. By comparing the value of specific and neurohumoral indices it was revealed that the neurohumoral shifts preceded the maximal development of the immune response. On the example of studying the catecholamine dynamics the opinion on a close association between the state of the regulatory mechanisms and the effector formations responsible for the formation of specific immunological reactions was confirmed. It is suggested that a full-value immunological response developed on condition of activation of the sympathico-adrenal system.
1971
Kanarek, D.J., Zwi, S., Milne, F.J. & Goldman, H.I. Alpha-1-antitrypsin deficiency. S Afr Med J 45, 6, 144-6 (1971).
Badenoch, J. The sulphonamides in the management of disease of the gut. Practitioner 206, 231, 12-5 (1971).

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