@article {1308851,
	title = {An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resistance},
	journal = {Cell},
	volume = {173},
	number = {3},
	year = {2018},
	pages = {649-664},
	abstract = {Resistance to chemotherapy plays a significant role\&nbsp;in cancer mortality. To identify genetic units affecting sensitivity to cytarabine, the mainstay of treatment for acute myeloid leukemia (AML), we\&nbsp;developed a comprehensive and integrated genome-wide platform based on a dual protein-coding and non-coding integrated CRISPRa screening (DICaS). Putative resistance genes were initially identified using pharmacogenetic data from 760 human pan-cancer cell lines. Subsequently, genome scale functional characterization of both coding and long non-coding RNA (lncRNA) genes by CRISPR activation was performed. For lncRNA functional assessment, we developed a CRISPR activation of lncRNA (CaLR) strategy, targeting 14,701 lncRNA genes. Computational and functional analysis identified novel cell-cycle, survival/apoptosis, and cancer signaling genes. Furthermore, transcriptional activation of the GAS6-AS2 lncRNA, identified in our analysis, leads to hyperactivation of the GAS6/TAM pathway, a resistance mechanism in multiple cancers including AML. Thus, DICaS represents a novel and powerful approach to identify integrated coding and non-coding pathways of therapeutic relevance.},
	url = {http://www.cell.com/cell/fulltext/S0092-8674(18)30384-2},
	author = {Assaf C. Bester and Jonathan D. Lee and Chavez, Alejandro and Yu-Ru Lee and Daphna Nachmani and Vora, Suhani and Joshua Victor and Martin Sauvageau and Emanuele Monteleone and Rinn, John L and Paolo Provero and Church, George M and Clohessy, John G and Pier Paolo Pandofi}
}
