@article {1172241,
	title = {Subtilopeptidase A isoenzyme system. Interaction with serum components and its importance for quantitative immunoelectrophoresis},
	journal = {Biochem J},
	volume = {151},
	number = {1},
	year = {1975},
	month = {1975 Oct},
	pages = {149-55},
	abstract = {A method was developed which involved electroimmunoassay and crossed immunoelectrophoresis of subtilopeptidase A (EC 3.4.21.14). Initial trials with unfractionated antiserum were not successful and interaction of the enzyme with non-immunoglobulin serum components were shown to be the cause of the failures. Quantitative immunoelectrophoresis was possible when purified immunoglobulins were used. A pH of 6.5 (lower than the usual pH 8.6) was necessary to obtain a proper baseline definition. Subtilopeptidase A was confirmed as a multiple isoenzyme system. Qualitative inter-batch variations were detected. Di-isopropyl phosphorofluoridate inhibition altered the electrophoretic pattern, but no loss of antigenic determinants was observed.},
	keywords = {Animals, Humans, Hydrogen-Ion Concentration, Immunoassay, Immunoelectrophoresis, Two-Dimensional, Immunoglobulins, Isoenzymes, Isoflurophate, Rabbits, Subtilisins},
	issn = {0264-6021},
	author = {Verbruggen, R}
}
