@article {1118456,
	title = {Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq},
	journal = {Science},
	volume = {355},
	number = {6332},
	year = {2017},
	month = {2017 Mar 31},
	abstract = {Tumor subclasses differ according to the genotypes and phenotypes of malignant cells as well as the composition of the tumor microenvironment (TME). We dissected these influences in isocitrate dehydrogenase (IDH)-mutant gliomas by combining 14,226 single-cell RNA sequencing (RNA-seq) profiles from 16 patient samples with bulk RNA-seq profiles from 165 patient samples. Differences in bulk profiles between IDH-mutant astrocytoma and oligodendroglioma can be primarily explained by distinct TME and signature genetic events, whereas both tumor types share similar developmental hierarchies and lineages of glial differentiation. As tumor grade increases, we find enhanced proliferation of malignant cells, larger pools of undifferentiated glioma cells, and an increase in macrophage over microglia expression programs in TME. Our work provides a unifying model for IDH-mutant gliomas and a general framework for dissecting the differences among human tumor subclasses.},
	issn = {1095-9203},
	doi = {10.1126/science.aai8478},
	url = {http://science.sciencemag.org/content/355/6332/eaai8478.long},
	author = {Venteicher, Andrew S and Itay Tirosh and Hebert, Christine and Yizhak, Keren and Neftel, Cyril and Filbin, Mariella G and Hovestadt, Volker and Escalante, Leah E and Shaw, McKenzie L and Rodman, Christopher and Gillespie, Shawn M and Dionne, Danielle and Luo, Christina C and Ravichandran, Hiranmayi and Mylvaganam, Ravindra and Mount, Christopher and Onozato, Maristela L and Nahed, Brian V and Wakimoto, Hiroaki and Curry, William T and Iafrate, A John and Rivera, Miguel N and Frosch, Matthew P and Golub, Todd R and Brastianos, Priscilla K and Getz, Gad and Patel, Anoop P and Monje, Michelle and Cahill, Daniel P and Rozenblatt-Rosen, Orit and Louis, David N and Bernstein, Bradley E and Regev, Aviv and Suv{\`a}, Mario L}
}
